Coding

Part:BBa_K2287026:Design

Designed by: Jiang Wu   Group: iGEM17_UCAS   (2017-10-20)


purC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence from E.coli harbors an illegal EcoRI site and is depleted by point mutation. No further optimization for expression in E.coli is needed.


Source

This sequence can directly obtained by PCR from genomic DNA of E.coli strain MG1655.

References